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Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

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Abstract

Gene therapy is a promising technology for the treatment of several acquired and inherited diseases. However, for gene therapy to be a commercial and clinical success, scalable cell culture processes must be in place to produce the required amount of viral vectors to meet market demand. Each type of vector has its own distinct characteristics and consequently its own challenges for production. This article reviews the current technology that has been developed for the efficient, large-scale manufacture of retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus vectors.

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Authors and Affiliations

  1. Department of Agricultural and Biological Engineering, Mississippi State University, 39762, Mississippi State, MS, USA

    James N. Warnock

  2. Généthon, 1bis Rue de l’Internationale, BP 60, F-91002, Evry-Cedex, France

    Otto-Wilhelm Merten

  3. School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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  1. James N. Warnock
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  2. Otto-Wilhelm Merten
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  3. Mohamed Al-Rubeai
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Correspondence to Mohamed Al-Rubeai.

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Warnock, J.N., Merten, OW. & Al-Rubeai, M. Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes. Cytotechnology 50, 141–162 (2006). https://doi.org/10.1007/s10616-005-5507-z

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