Summary
The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell number with increasing concentration of Pluronic. Such growth inhibition was found to be a result of lowering the rate of DNA synthesis.
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Al-Rubeai, M., Emery, A.N. & Chalder, S. The effect of Pluronic F-68 on hybridoma cells in continuous culture. Appl Microbiol Biotechnol 37, 44–45 (1992). https://doi.org/10.1007/BF00174201
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DOI: https://doi.org/10.1007/BF00174201