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Hosting the plant cells in vitro: recent trends in bioreactors

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Abstract

Biotechnological production of high-value metabolites and therapeutic proteins by plant in vitro systems has been considered as an attractive alternative of classical technologies. Numerous proof-of-concept studies have illustrated the feasibility of scaling up plant in vitro system-based processes while keeping their biosynthetic potential. Moreover, several commercial processes have been established so far. Though the progress on the field is still limited, in the recent years several bioreactor configurations has been developed (e.g., so-called single-use bioreactors) and successfully adapted for growing plant cells in vitro. This review highlights recent progress and limitations in the bioreactors for plant cells and outlines future perspectives for wider industrialization of plant in vitro systems as “green cell factories” for sustainable production of value-added molecules.

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Acknowledgments

Financial support from the National Science Fund of Bulgaria (grant DO-02-261/2008 to MIG) is greatly appreciated. JJZ appreciates the financial support from the National Natural Science Foundation of China (NSFC) and the SJTU University Distinguished Professorship program as well as excellent collaboration with Prof. Xuhong Qian (ECUST) on novel synthetic elicitors.

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  1. Department of Applied Biotechnologies, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000, Plovdiv, Bulgaria

    Milen I. Georgiev

  2. School of Life Sciences and Facility Management, Institute of Biotechnology, Zurich University of Applied Sciences and Arts, Grüental, 8820, Wädenswil, Switzerland

    Regine Eibl

  3. State Key Laboratory of Microbial Metabolism, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dong-chuan Road, Shanghai, 200240, China

    Jian-Jiang Zhong

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  1. Milen I. Georgiev
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  2. Regine Eibl
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  3. Jian-Jiang Zhong
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Correspondence to Milen I. Georgiev.

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Georgiev, M.I., Eibl, R. & Zhong, JJ. Hosting the plant cells in vitro: recent trends in bioreactors. Appl Microbiol Biotechnol 97, 3787–3800 (2013). https://doi.org/10.1007/s00253-013-4817-x

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