Elsevier

Plant Science

Volume 242, January 2016, Pages 203-213
Plant Science

Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes

https://doi.org/10.1016/j.plantsci.2015.08.013Get rights and content
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Highlights

  • Two molecular breeding approaches namely MABC and MAS were used to enhance oil quality in peanut.

  • Developed high oleate introgression lines; 27 with high and 28 with low oil content.

  • All the selected introgression lines had low linoleic and palmitic acid content.

  • No negative association between high oil content and high oleic acid.

  • Oleic acid increased by 0.5–1.1 folds at par with the donor parent, SunOleic 95R.

Abstract

High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 (ahFAD2A) and b09 (ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142 and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5–1.1 folds, with concomitant reduction of linoleic acid by 0.4–1.0 folds and palmitic acid by 0.1–0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53–58%), and 28 ILs with low oil content (42–50%) were selected that may be released for cultivation upon further evaluation.

Abbreviations

MABC
marker-assisted backcrossing
MAS
marker-assisted selection
ILs
introgression lines
CVD
cardiovascular diseases
LDL
low density lipoproteins
HDL
high density lipoproteins
FAD
fatty acid desaturase
CAPS
cleaved amplified polymorphic sequences
AS-PCR
allele specific polymerase chain reaction
ICGV
ICRISAT groundnut variety

Keywords

Peanut
Oil content
Oleic acid
Oil quality
Marker-assisted backcrossing
Marker-assisted selection

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1

These authors contributed equally.