Studies of Pluripotency in Embryonic Stem Cells and Induced Pluripotent Stem Cells

1. Front Matter

   Pages i-xv

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2. Introduction

   • Xiaoyang Zhao

   Pages 1-26

3. Establishment of ESC Lines Derived from Mice, Rats, and Primate

   • Xiaoyang Zhao

   Pages 27-39

4. Establishment of Highly Efficient Somatic Cell Reprogramming System to Generate iPSC Lines

   • Xiaoyang Zhao

   Pages 41-52

5. Pluripotency of iPSC and the Underlining Mechanism

   • Xiaoyang Zhao

   Pages 53-74

6. Developmental Potential of Mouse iPSC

   • Xiaoyang Zhao

   Pages 75-89

7. Conclusion

   • Xiaoyang Zhao

   Pages 91-92

Stem cells have the ability to differentiate into all types of cells within the body, thus have great therapeutic potential for regenerative medicine to treat complicated disorders, like Parkinson’s disease and spinal cord injury. There will also be many applications in drug development. However, several roadblocks, such as safety issues and low efficiency of pluripotent stem cell (PSC) line derivation need to be resolved before their clinical application. This thesis focuses on these two areas, so as to find methods to overcome the limitation. It covers deriving embryonic stem cells (ESCs) from several different species and reports an efficient system to generate induced pluripotent stem cells (iPSCs), and the first iPSC mice in the world. The results in this thesis confirm that somatic cells can be fully reprogrammed with the four Yamanaka factors. In addition, we have found that the Dlk1-Dio3 region can be a potential molecular marker to distinguish the fully reprogrammed iPSCs from partially reprogrammed ones. All of these results will help improve the safety of PSCs in the clinical applications and increase the current low induction efficiency of their production.

• Dlk1-Dio3 region
• embryonic stem cells
• induced pluripotent stem cells
• pluripotency
• tetraploid complementation
• Cell culture

• Institute of Zoology Chinese Academy of Sciences, Beijing, China

  Xiaoyang Zhao

Xiaoyang Zhao obtained his Ph.D. from the Institute of Zoology, Chinese Academy of Science (CAS). He was supervised by Professor Qi Zhou, who is one of the most famous researchers in stem cells and regenerative medicine. During his Ph.D. study, Xiaoyang Zhao published 9 research articles as the first author in well-known international journals, such as Nature, Cell Research and the Journal of Biological Chemistry. He received the Excellent Graduates award from the Institute of Zoology, CAS and the first ISTT Young Investigator Award from the International Society for Transgenic Technologies (ISTT) in 2011. Dr. Xiaoyang Zhao is currently a principle investigator at the Institute of Zoology, CAS.

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