Animal Cell Culture

1. Front Matter

   Pages i-xii

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2. Cell Line Development

   • Hansjoerg Hauser

   Pages 1-25

3. Transient Recombinant Protein Expression in Mammalian Cells

   • Volker Jäger, Konrad Büssow, Thomas Schirrmann

   Pages 27-64

4. Production of Antibodies in Hybridoma and Non-hybridoma Cell Lines

   • Susan McDonnell

   Pages 65-88

5. Bioreactors for Mammalian Cells

   • R. Pörtner

   Pages 89-135

6. Mass Transfer and Mixing Across the Scales in Animal Cell Culture

   • Alvin W. Nienow

   Pages 137-167

7. Hydrodynamic Damage to Animal Cells

   • Jeffrey J. Chalmers, Ningning Ma

   Pages 169-183

8. Monitoring of Cell Culture

   • Sebastian Schwamb, Robert Puskeiler, Philipp Wiedemann

   Pages 185-221

9. Serum and Protein Free Media

   • Michael Butler

   Pages 223-236

10. Glycosylation in Cell Culture

    • Maureen Spearman, Michael Butler

    Pages 237-258

11. Modelling of Mammalian Cell Cultures

    • Nishikant P. Shirsat, Niall J. English, Brian Glennon, Mohamed Al-Rubeai

    Pages 259-326

12. Mammalian Cell Line Selection Strategies for High-Producers

    • Darrin Kuystermans, Mohamed Al-Rubeai

    Pages 327-372

13. Building a Cell Culture Process with Stable Foundations: Searching for Certainty in an Uncertain World

    • Peter M. O’Callaghan, Andrew J. Racher

    Pages 373-406

14. Perfusion Processes

    • Véronique Chotteau

    Pages 407-443

15. Single-Use Bioreactors for Animal and Human Cells

    • Stephan C. Kaiser, Matthias Kraume, Dieter Eibl, Regine Eibl

    Pages 445-500

16. An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS)

    • Laura A. Palomares, Mauricio Realpe, Octavio T. Ramírez

    Pages 501-519

17. Metabolic Flux Analysis: A Powerful Tool in Animal Cell Culture

    • João V. Sá, Tiago M. Duarte, Manuel J. T. Carrondo, Paula M. Alves, Ana P. Teixeira

    Pages 521-539

18. Cell Immobilization for the Production of Viral Vaccines

    • Ana Carina Silva, António Roldão, Ana Teixeira, Paulo Fernandes, Marcos F. Q. Sousa, Paula M. Alves

    Pages 541-563

19. Cell Engineering for Therapeutic Protein Production

    • Eric Baek, Che Lin Kim, Jin Hyoung Park, Gyun Min Lee

    Pages 565-590

20. Proteomics in Cell Culture: From Genomics to Combined ‘Omics for Cell Line Engineering and Bioprocess Development

    • Kelley Heffner, Christian Schroeder Kaas, Amit Kumar, Deniz Baycin-Hizal, Michael Betenbaugh

    Pages 591-614

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production.  Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

• Animal Cell Culture
• Biologics
• Biopharmaceuticals
• Bioprocessing
• Therapeutic Products
• Cell culture

• University College Dublin, Dublin, Ireland

  Mohamed Al-Rubeai

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