Engineering of Halogenases towards Synthetic Applications

1. Front Matter

   Pages I-XII

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2. Introduction

   • Hannah Minges

   Pages 13-24

3. Motivation

   • Hannah Minges

   Pages 25-25

4. Material and Methods

   • Hannah Minges

   Pages 27-57

5. Directed evolution of Thal: Results

   • Hannah Minges

   Pages 59-79

6. Directed evolution of Thal: Discussion

   • Hannah Minges

   Pages 81-87

7. Establishment of a marine brominase: Results

   • Hannah Minges

   Pages 89-108

8. Establishment of a marine brominase

   • Hannah Minges

   Pages 109-119

9. Outlook

   • Hannah Minges

   Pages 121-122

10. Summary

    • Hannah Minges

    Pages 123-126

11. Back Matter

    Pages 127-128

    PDF

Hannah Minges focuses on the investigation of two different FAD-dependent halogenases in order to analyze and improve their applicability for chemoenzymatic approaches in chemistry. Owing to beneficial features, like high selectivity and benign reaction conditions, nature’s toolkit for halogenation provides several advantages, whereas conventional chemical strategies require hazardous reagents and suffer from low selectivity. Therefore, enzymatic halogenation arises as promising alternative in the synthesis of valuable chemicals. One project focuses on the generation of a thermostable variant of the tryptophan halogenase Thal by means of directed evolution. The second project deals with the investigation of the marine halogenase Bmp5. This enzyme is of synthetic interest because it preferably introduces bromine into phenol compounds, whereas chlorination cannot take place. 

• Thal
• Bmp5
• Directed Evolution
• Enzyme
• Biocatalysis

• Bielefeld, Germany

  Hannah Minges

Hannah Minges, a Ph.D student at Bielefeld University, works on the optimization of tryptophan halogenases by means of directed evolution.

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