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Efficient plant regeneration from cell suspension protoplasts of the woody medicinal plant Solanum dulcamara L. (bittersweet, woody nightshade)

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Abstract

Large populations of viable protoplasts were released from suspension cultured cells of the woody medicinal plant Solanum dulcamara (bittersweet, woody nightshade) when the cells were harvested 3 to 7 months after culture initiation and 4 to 5 days after transfer to fresh medium. A Bio-Gel p6 purified enzyme mixture enhanced the protoplast plating efficiency 6 fold compared to the unpurified mixture, without affecting protoplast yield. Agarose-solidified medium markedly improved protoplast division and colony formation, and enabled protoplasts to be plated at lower densities than in liquid medium. All protoplast-derived tissues produced shoots on MS based medium with 1.0 mgl-1 zeatin. Shoots rooted readily on medium lacking phytohormones. Cytological examination revealed high chromosome stability of suspension cultured cells, of plants derived from such cells, and of protoplast-derived plants. The implication of these results is discussed in relation to the genetic manipulation of this pharmaceutically important plant.

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    P. K. Chand, M. R. Davey & J. B. Power

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  1. P. K. Chand
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  2. M. R. Davey
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  3. J. B. Power
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Chand, P.K., Davey, M.R. & Power, J.B. Efficient plant regeneration from cell suspension protoplasts of the woody medicinal plant Solanum dulcamara L. (bittersweet, woody nightshade). Plant Cell Tiss Organ Cult 22, 119–125 (1990). https://doi.org/10.1007/BF00043687

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