Authors:
- Assumes no prior knowledge on the part of the reader
- Easy to understand, concise writing style teaches novices to read and speak the "language" of gene cloning
- Includes sample applications of gene cloning in food science, nutrition, and biomedicine, including gene therapy and cloning
- End-of-chapter exercises and reading assignments facilitate self-study
- New to this edition are extensive updates on the current state of whole genome sequencing as well as personalized medicine and gene therapy, as well as new methods of gene editing and methodologies for locating disease genes
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Table of contents (24 chapters)
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Front Matter
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Fundamentals of Genetic Processes
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Front Matter
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Techniques and Strategies of Gene Cloning
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Front Matter
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Impact of Gene Cloning: Applications in Agriculture
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Front Matter
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About this book
Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas.
The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.
Keywords
- the genetic process
- reading nucleotide gene sequence
- Enzymes cloning gene
- techniques cloning gene
- cloning vectors host cells
- organization of genes
- cloning vectors gene cells
- isolating gene cloning
- transgenic crops herbicide resistance
- growth enhancement transgenic fish
- microbial produciton human insulin
- production recombinant human insulin
- microbial recombinant human insulin
- dna typing gene cloning
- dna typing gene
Authors and Affiliations
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Western Regional Research Center, Albany, USA
Dominic W. S. Wong
About the author
Bibliographic Information
Book Title: The ABCs of Gene Cloning
Authors: Dominic W. S. Wong
DOI: https://doi.org/10.1007/978-3-319-77982-9
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing AG, part of Springer Nature 2018
Softcover ISBN: 978-3-319-77762-7Published: 28 July 2018
eBook ISBN: 978-3-319-77982-9Published: 26 May 2018
Edition Number: 3
Number of Pages: XIX, 257
Number of Illustrations: 171 b/w illustrations, 1 illustrations in colour
Topics: Human Genetics, Food Science, Plant Physiology, Zoology