Advances in New Technology for Targeted Modification of Plant Genomes

1. Front Matter

   Pages i-viii

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2. Double-Strand Break Repair and Its Application to Genome Engineering in Plants

   • Holger Puchta, Friedrich Fauser

   Pages 1-20

3. Engineering Meganuclease for Precise Plant Genome Modification

   • Fayza Daboussi, Thomas J. Stoddard, Feng Zhang

   Pages 21-38

4. High Efficient Genome Modification by Designed Zinc Finger Nuclease

   • Yiping Qi

   Pages 39-53

5. Engineered TAL Effector Proteins: Versatile Reagents for Manipulating Plant Genomes

   • Michelle Christian, Daniel F. Voytas

   Pages 55-72

6. Oligo-Mediated Targeted Gene Editing

   • Greg F. W. Gocal, Christian Schöpke, Peter R. Beetham

   Pages 73-89

7. Gene Targeting in Crop Species with Effective Selection Systems

   • Takaki Yamauchi, Shigeru Iida

   Pages 91-111

8. Recombinase Technology for Precise Genome Engineering

   • James G. Thomson, Ann Blechl

   Pages 113-144

9. Developing CRISPR Technology in Major Crop Plants

   • Kunling Chen, Caixia Gao

   Pages 145-159

10. Back Matter

    Pages 161-166

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Over the past 50 years, biotechnology has been the major driving force for increasing crop productivity. Particularly, advances in plant genetic engineering technologies have opened up vast new opportunities for plant researchers and breeders to create new crop varieties with desirable traits. Recent development of precise genome modification methods, such as targeted gene knock-out/knock-in and precise gene replacement, moves genetic engineering to another level and offers even more potentials for improving crop production. The work provides an overview of the latest advances on precise genomic engineering technologies in plants. Topics include recombinase and engineered nucleases-mediated targeted modification, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. Finally, challenges and impacts of the new technologies on present regulations for genetic modification organisms (GMOs) will be discussed.

• Crop Production
• Gene Targeting
• Genome Modification
• Mutagenesis
• New Technology

• Cellectis Plant Sciences, New Brighton, USA

  Feng Zhang

• Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany

  Holger Puchta

• Crop Improvement and Genetics Unit, USDA Agricultural Research Service, Western Regional Research Center, Albany, USA

  James G. Thomson

Feng Zhang, Research Director, Cellectis plant sciences

Holger Puchta, Professor, University of Karlsruhe

James Thompson, Principle Investigator, USDA

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