1.21 - Modes of Culture/Animal Cells

https://doi.org/10.1016/B978-0-08-088504-9.00033-7Get rights and content

Abstract

Based on the control scheme of nutrients and metabolites, there are many modes of cell culture processes, including batch, repeated-batch, fed-batch, chemostat, and perfusion, among which batch, fed-batch, and perfusion modes are used for industrial-scale biologics production. Batch is the simplest with little operation control of nutrients and metabolites, whereas perfusion is the most complex with intensive control. Generally, high-level control leads to high productivity, but with a tradeoff of high complexity, increasing risk of failure, and developmental time and cost. The choice of the culture mode is largely dependent on process capacity and quality requirements. Different culture modes are well distinguishable in cell growth, nutrient consumption, and product formation kinetics. Understanding the kinetics is the starting point for process development and improvement. The development of more sophisticated fed-batch and perfusion cultures is rooted in the understanding of the process established in batch culture. Batch culture is the indispensable first step for establishing a proper cell maintenance schedule and for further developing other culture modes with more complexity and higher performance. This article provides detailed discussions on different culture modes and associated culture kinetics and process design and operation considerations in the biotechnology industry.

References (0)

Cited by (0)

Xudong Zhang received his BS degree in biotechnology from Peking University, Beijing, China in 2001 and his doctorate degree in biochemistry from the Ohio State University in 2008. He is currently a Sr. scientist in Cell Culture Process Development at Diosynth Biotechnology. His current work focuses on optimization and characterization of cell culture processes using structured experimentation, characterization of bioreactors by scalability study, and evaluation of changing technologies in the biopharmaceutical industry.

Yuan Wen received his BS degree in bioengineering from Zhejiong University, China in 2003 and PhD degree in chemical and biomolecular engineering from the Ohio State University in 2009. His graduate research focused on microfluidic and microscale high-throughput cell culture systems for cell-based assays and bioprocess development. He currently works as a staff scientist for PD Direct® Bioprocess Services at Life Technologies Corporation.

Shang-Tian Yang is a professor of chemical and biomolecular engineering at the Ohio State University, where he has been on the faculty since 1985 and currently teaches courses in biotechnology and bioprocess engineering. Dr. Yang is a co-founder of two biotechnology startup companies. He is also the director of Ohio Bioprocessing Research Consortium and has worked with many companies in commercial technology development. Dr. Yang has broad research interests in bioengineering. His current research involves biocatalysis, cell culture, tissue engineering, functional genomics, and microfluidic biochips for high-throughput cell-based assays and biodiagnostics. He has more than 150 scientific publications and 13 patents in the bioengineering field. Dr. Yang received his BS degree in Agricultural Chemistry from National Taiwan University and MS and PhD degrees in biochemical engineering from Purdue University. He is an elected fellow of American Institute of Medical and Biological Engineering and an active member of American Institute of Chemical Engineers (AIChE) and American Chemical Society (ACS). He also serves as an associate editor for the journal Process Biochemistry.

View full text