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Electroporation stimulates tranformation of freshly isolated cell suspension protoplasts ofSolanum dulcamara byAgrobacterium

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Summary

Freshly isolated cell suspension protoplasts ofSolanum dulcamara were mixed withAgrobacterium rhizogenes, allowed to settle for 2 h, exposed to electrical pulses and further incubated for 2h. Two pulses of 600 V cm−1 for 2 msec separated by 15 sec produced transformed colonies at relative and absolute transformation frequencies which were 3–4 and 10 fold greater than those obtained by co-cultivation of 3 days old protoplast-derived cells with bacteria. Transformed colonies were not produced when freshly isolated protoplasts were mixed withAgrobacterium but not electroporated. Biochemical analysis confirmed the transgenic nature of plants regenerated from protoplast-derived tissues.

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Abbreviations

MS:

Murashige and Skoog (1962)

NPTII:

Neomycin phosphotransferase II

SDS:

Sodium dodecylsulphate

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, United Kingdom

    P. K. Chand, E. L. Rech, T. J. Golds, J. B. Power & M. R. Davey

Authors
  1. P. K. Chand
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  2. E. L. Rech
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  3. T. J. Golds
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  4. J. B. Power
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  5. M. R. Davey
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Communicated by I. Potrykus

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Chand, P.K., Rech, E.L., Golds, T.J. et al. Electroporation stimulates tranformation of freshly isolated cell suspension protoplasts ofSolanum dulcamara byAgrobacterium . Plant Cell Reports 8, 86–89 (1989). https://doi.org/10.1007/BF00716845

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  • DOI: https://doi.org/10.1007/BF00716845

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