Abstract
Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.
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This work was supported by the Takeda Science Foundation and Grants-in-Aid for Scientific Research (16H05377, 16H06403, and 16H06400).
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All study participants and their legal guardians provided written informed consent prior to enrollment. This study was approved by the appropriate Ethics Committee of the Nara Medical University (No. 1319-5) and was performed in accordance with the Declaration of Helsinki.
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Editor: Tetsuji Okamoto
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Toritsuka, M., Makinodan, M., Yamauchi, T. et al. Altered gene expression in lymphoblastoid cell lines after subculture. In Vitro Cell.Dev.Biol.-Animal 54, 523–527 (2018). https://doi.org/10.1007/s11626-018-0267-1
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DOI: https://doi.org/10.1007/s11626-018-0267-1